Kinetic and Chemical Mechanism of Malate Synthase fromMycobacterium tuberculosis
نویسندگان
چکیده
منابع مشابه
Biochemical and structural studies of malate synthase from Mycobacterium tuberculosis.
Establishment or maintenance of a persistent infection by Mycobacterium tuberculosis requires the glyoxylate pathway. This is a bypass of the tricarboxylic acid cycle in which isocitrate lyase and malate synthase (GlcB) catalyze the net incorporation of carbon during growth of microorganisms on acetate or fatty acids as the primary carbon source. The glcB gene from M. tuberculosis, which encode...
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The catalytic mechanism of 3-deoxy-D-manno-2-octulosonate-8-phosphate (Kdo8P) synthase from Escherichia coli was investigated under pre-steady-state conditions using rapid chemical quench flow methods. The results suggest the formation of acyclic bisphosphate 1 as a reaction intermediate. © 1997 Elsevier Science Ltd. 3-Deoxy-D-manno-2-octulosonate-8-phosphate (Kdo8P) synthase (EC 4.1.2.16) is a...
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catalyzed by glycogen synthase D (UDP-glucose:glycogen o-4-glucosyltransferase, EC 2.4.1.11) was studied, using r4C isotope transfer rates. The reaction rates were determined at varying concentrations of either substrate at constant amounts of the other substrate. The inhibition patterns of UDP as well as the activation patterns of the activator glucose 6-phosphate toward either substrate were ...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2011
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi2007299